Smart Life Sciences Strategies for maximium Research Impact
Model Development, Characterisation and Depolyment Strategies.
Research Model Development
Select the right cell, tissue, organ or animal models
Develop new models of health and disease
In vitro, ex-vivo and in vivo model assay optimisation
Research Model Characterisation
Deeply phenotype your experimental models
Optimise and increase throughput
Validate the model for your use cases
Research Model Deployment
Design of experiments
Discovery workflows
Presentation and publication strategies
Expertise
In vitro model solutions
Fresh hepatocyte isolation. Cryopreserved Hepatocytes, Stellate cells, Kupffer cells, Stem cell-derived hepatocyte-like cells
Established cell lines: Hepatic, Pancreatic, Ovarian, Kidney, Fibroblast, Gastric cancer, lung lines and more
Ex vivo Human Liver and tumour slice models
Hanging drop spheroids, perfused bioreactors, and Air-Liquid interface models
Model Characterisation
Application of global -omics approaches such as labelled and unlabelled proteomics or RNAseq
Microscopy; Bright-field, Fluorescent, Confocal, SEM and TEM
Standard laboratory techniques and assays including Western Blotting, ELISA, FACS analysis and Immunohistochemical staining.
Cellular fractionation: liver microsomes, cytosol, nuclear extracts
qPCR, mRNA knockdown, molecular cloning, DNA transfection, bacterial transformation, bacterial protein expression, affinity purification techniques
Colorimetric, fluorescent, UV absorbance or luminescent assays using laboratory reagents or commercial kits
In vivo model solutions
Syngeneic and xenograft tumour models
In vivo imaging (IVIS)
Large animal safety models
Drug metabolism
In vitro and in vivo dosing and sampling strategies
In vitro and in vivo cytochrome P450 induction/inhibition
Serum/media whole cell or cell fraction metabolism studies
Quantification of metabolism using biochemical assay kits, HPLC or Mass Spectrometry